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pmtor ser2448  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pmtor ser2448
    Pmtor Ser2448, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 3437 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmtor+ser2448/Phospho-mTOR+(Ser2448)+XP+Rabbit+mAb/pmc13049662-40-1-4
    Average 97 stars, based on 3437 article reviews
    pmtor ser2448 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Western Blot:

    Article Title: Uncoupling of Akt and mTOR signaling drives resistance to Akt inhibition in PTEN loss prostate cancers.
    Article Snippet: .. The following antibodies were used for Western blotting: PSA (5365S), pAkt (Ser473) (Cell Signaling Technology, 4060L), pAkt (Thr308) (Cell Signaling Technology, 4056S), pEGFR (Cell Signaling Technology, 3777S), p- p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP (Cell Signaling Technology, 4370S), pMEK1/2 (Ser217/221) (41G9) (Cell Signaling Technology, 9154S), pS6K (Thr389) (Cell Signaling Technology, 9205L), pS6 (Ser235/236) (Cell Signaling Technology, 4856S), PTEN (Cell Signaling Technology, 9188L), pPRAS40 (Thr246) (Cell Signaling Technology, 2997S), p4EBP1 (Thr37/46) (Cell Signaling Technology, 2855L), pmTOR (Ser2448) (Cell Signaling Technology, 5536S), TSC2 (Cell Signaling Technology, 4308S), HSP90 (Cell Signaling Technology, 4875S), GCN2 (Cell Signaling Technology, 3302S), pEIF2α (Ser51) (Cell Signaling Technology, 3398S), LC3A/B (Cell Signaling Technology, 12741S), LC3B (Cell Signaling Technology, 277S), β- actin (Cell Signaling Technology, 4970S), NPRL3 (Thermo Fisher, PA5- 52849), ATG4C (Cell Signaling Technology, 5262S), BIP (Cell Signaling Technology, 3177S), AR (Abcam, AB108341), FKBP5 (Cell Signaling Technology, 12210S), STEAP1 (Santa Cruz Biotechnology, SC- 271872), and PMEPA1 (Santa Cruz Biotechnology, SC- 293372). ..

    other:

    Article Title: AMPK inhibition and elevated angiogenin are associated with tRNA fragmentation in the male germline exposed to a high-fat diet
    Article Snippet: , pmTOR (Ser2448) , Cell signaling technology , 5536 , 1:1000 in BSA.

    Incubation:

    Article Title: Liver impact of growth hormone (GH) intermittent treatment during the growth period in mice.
    Article Snippet: Liver impact of prolonged GH-treatment given to non-GH-deficient growing mice between the third and eighth week of life was evaluated in both sexes.. Tissues were collected 6 h after last dose or four weeks later.. Somatometric, biochemical, histological, immunohistochemical, RT-qPCR and immunoblotting determinations were

    Article Title: A modular microfluidic platform to study how fluid shear stress alters estrogen receptor phenotype in ER+ breast cancer cells
    Article Snippet: The blots were blocked by incubation in EveryBlot blocking buffer (BIO-RAD 12010020) per manufacturer’s protocol. .. After blocking, the membrane was incubated with primary antibody overnight at 4°C for p-AKT (Ser473) (Cell Signaling Technologies, #4060), p-ERα (Ser167) (Cell Signaling Technologies, #64508), pmTOR (Ser2448) (Cell Signaling Technologies, #5536), p-STAT3 (Tyr705) (Cell Signaling Technologies, #9145) and p-ERK1/2 (Thr202/Tyr204) (Cell Signaling Technologies, #9101). ..

    Ubiquitin Proteomics:

    Article Title: GL261 glioblastoma induces delayed body weight gain and stunted skeletal muscle growth in young mice
    Article Snippet: Optical density measurements were taken using the ImageJ Analyzer software. .. Antibodies used were pSTAT1 (#9167), STAT1 (#9712), pSTAT3 Y705 (#9145), STAT3 (#8768), pAkt S473 (#4060), Akt (#9272), pP38 MAPK (T180/Y182) (#4511), P38 MAPK (#9212), pERK1/2 (#4370), ERK1/2 (#4695), p4EBP1 (#2855), 4EBP1 (#9644), VDAC (#4866), pFoxO3a Ser253 (#9466), FoxO3a (#12829), OPA1 (#80471), pmTOR Ser2448 (#5536), mTOR (#2983), H3 (#4499), LDH (#2012), Cytochrome C (#11940), Tom20 (#42406), Mitofusin 2 (#9482) and Ubiquitin (#3933) from Cell Signaling Technologies, α-Tubulin (#12G10) from Developmental Studies Hybridoma Bank, LC3BI/II (#L7643) from Sigma, PGC-1α (AB3242) from EMD Millipore, puromycin (EQ0001) from Kerafast, and COX-IV (ab14744) from Abcam. ..

    Article Title: Targeting the IRS1 macromolecular signaling node by Trienomycin a triggers cytoprotective autophagy in pancreatic adenocarcinoma.
    Article Snippet: After blocking with Trisbuffered saline with 0.05 % Tween-20 (TBST) supplemented with 5 % non-fat dry milk, the NC membranes were incubated with specific primary antibodies and secondary antibody (Cell Signaling Technology) sequentially, then the membranes were visualized by using ECL solution and scanned by using the Imaging System (Alliance Q9 Advanced, Uvitec, England). .. Primary antibodies used in the paper included pmTOR (Ser2448) (CST, 5563), p-ULK1 (Ser757) (CST, 14202), p-PI3K (Tyr458) (Affinity, AF3242), p-Akt (Ser473) (Proteintech 28,731), mTOR (CST, 2972), PI3K (Proteintech, 60225), Akt (Proteintech, 60203), IRS1(Proteintech, 17509), Ubiquitin (Proteintech, 10201), FBXW8 (Santa, 514385), DYKDDDDK tag (Proteintech, 66008), MYC tag (Proteintech, 60003), Mouse IgG (Proteintech, SA00001–1-A), Rabbit IgG (Proteintech, SA00001–7 L) and β-Actin (Proteintech, 66009). .. PANC-1, MIA Paca-2, and BxPc-3 cells were seeded into 6-well plates and infected with Lenti-virus containing mCherry-Wassabi-LC3B (Beyotime) for 48 h, screened with puromycin to establish cell lines with stable expression of mCherry-Wassabi-LC3B protein.

    Pyrolysis Gas Chromatography:

    Article Title: GL261 glioblastoma induces delayed body weight gain and stunted skeletal muscle growth in young mice
    Article Snippet: Optical density measurements were taken using the ImageJ Analyzer software. .. Antibodies used were pSTAT1 (#9167), STAT1 (#9712), pSTAT3 Y705 (#9145), STAT3 (#8768), pAkt S473 (#4060), Akt (#9272), pP38 MAPK (T180/Y182) (#4511), P38 MAPK (#9212), pERK1/2 (#4370), ERK1/2 (#4695), p4EBP1 (#2855), 4EBP1 (#9644), VDAC (#4866), pFoxO3a Ser253 (#9466), FoxO3a (#12829), OPA1 (#80471), pmTOR Ser2448 (#5536), mTOR (#2983), H3 (#4499), LDH (#2012), Cytochrome C (#11940), Tom20 (#42406), Mitofusin 2 (#9482) and Ubiquitin (#3933) from Cell Signaling Technologies, α-Tubulin (#12G10) from Developmental Studies Hybridoma Bank, LC3BI/II (#L7643) from Sigma, PGC-1α (AB3242) from EMD Millipore, puromycin (EQ0001) from Kerafast, and COX-IV (ab14744) from Abcam. ..

    Blocking Assay:

    Article Title: A modular microfluidic platform to study how fluid shear stress alters estrogen receptor phenotype in ER+ breast cancer cells
    Article Snippet: The blots were blocked by incubation in EveryBlot blocking buffer (BIO-RAD 12010020) per manufacturer’s protocol. .. After blocking, the membrane was incubated with primary antibody overnight at 4°C for p-AKT (Ser473) (Cell Signaling Technologies, #4060), p-ERα (Ser167) (Cell Signaling Technologies, #64508), pmTOR (Ser2448) (Cell Signaling Technologies, #5536), p-STAT3 (Tyr705) (Cell Signaling Technologies, #9145) and p-ERK1/2 (Thr202/Tyr204) (Cell Signaling Technologies, #9101). ..

    Membrane:

    Article Title: A modular microfluidic platform to study how fluid shear stress alters estrogen receptor phenotype in ER+ breast cancer cells
    Article Snippet: The blots were blocked by incubation in EveryBlot blocking buffer (BIO-RAD 12010020) per manufacturer’s protocol. .. After blocking, the membrane was incubated with primary antibody overnight at 4°C for p-AKT (Ser473) (Cell Signaling Technologies, #4060), p-ERα (Ser167) (Cell Signaling Technologies, #64508), pmTOR (Ser2448) (Cell Signaling Technologies, #5536), p-STAT3 (Tyr705) (Cell Signaling Technologies, #9145) and p-ERK1/2 (Thr202/Tyr204) (Cell Signaling Technologies, #9101). ..



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